James Brownlow was the youngest son of James Brownlow and was mentioned in his will in 1740. James immegrated with his brother, William, to Charles Town, South Carolina, in December 1767. He was 30. He purchased several tracks of land in Craven County, South Carolina. The last record that mentions him is dated February 27, 1769, when he purchased 100 acres. He may have sold his land and moved to North Carolina with William's family. His is not known to have married or had children.