James Brownlow was the youngest son of James Brownlow and was mentioned in his will in 1740. James immigrated with his brother, William, to Charles Town, South Carolina, in December 1767. He was 30. He purchased several tracks of land in Craven County, South Carolina. He died in 1785 as James Patterson, his brother in law, was granted letters of administration on his estate in January of 1786 in Cumberland County, North Carolina. His is not known to have married or had children.